AnaSpec, Eurogentec’s newest North American division, is pleased to offer Eurogentec’s popular monoclonal antibody, anti-5-methylcytidine （Clone 33D3） for use in epigenetics research. Purified from ascites, this IgG1 / λ isotype antibody is supplied as a 1 mg/ml solution （in PBS） and is available in three sizes （0.1 mg, 0.5 mg and 1 mg）.

5-Methylcytidine is a modified base found in the DNA of plants and vertebrate. DNA methylation is a post-replication process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Alterations of DNA methylation pattern are associated with carcinogenesis. A global DNA hypomethylation is often detected in tumor tissues, associated with local hypermethylation sites. Since this antibody discriminates between the modified base, 5-MeCyd and its normal counterpart, cytidine; it is a useful tool for gene promoter methylation analysis.

The most published anti 5-Methylcytidine antibody available on the market, anti-5-Methylcyidine from Clone 33D3 can be used in applications such as Methylated DNA Immunoprecipitation （MeDIP）, MeDIP-on-chip assay, immunofluorescence, flow cytometry and immunohistochemistry. Histopathological analysis of tissue samples can be performed without having to destroy cells through DNA extraction procedures.

This antibody has been used to detect alterations in the urinary excretion of nucleosides from cancer patients, to visualize the distribution of methyl-rich regions along human chromosomes, to quantify in situ differences between normal and malignant cells from peripheral blood as well as on tissue sections.

The following papers are examples of recent publications citing the use of this antibody. To view a citation archive, please click here.

Jin, SG. et al. （2010）. Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine. Nucleic Acid Res. doi:10.1093/nar/gkq22.

Sanchez-Abarca, LI. et al. （2010）. Immunomodulatory effect of 5-azacytidine （5-azaC）: potential role in the transplantation setting. Blood 115: 107, doi: 10.1182/blood-2009-03-210393.

Wolk, M. & JE. Martin （2009）. Titrimetric immunohistochemical evaluation of DNA hypomethylation in uterine tumours. J. Clin. Pathol. 62:1039, doi:10.1136/jcp.2009.066613.

Peters, DD. et al. （2009）. Mammalian Genome 20: 664, doi: 10.1007/s00335-009-9227-0.

Mutskov, V. & G. Felsenfeld （2009）. The human insulin gene is part of a large open chromatin domain specific for human islets. Proc. Natl. Acad. Sci. 106: 17419, doi: 10.1073/pnas.0909288106.

Other epigenetics products include a wide selection of histone peptides, especially histone H3 peptides, SensoLyte® HDAC, LSD1 and SIRT1 assay kits.